Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP).

TitleSimultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP).
Publication TypeJournal Article
Year of Publication2014
AuthorsHsieh K, Mage PL, Csordas AT, Eisenstein M, H Soh T
JournalChem Commun (Camb)
Volume50
Issue28
Pagination3747-9
Date Published2014 Apr 11
ISSN1364-548X
Abstract

We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.

DOI10.1039/c4cc00540f
Alternate JournalChem. Commun. (Camb.)
PubMed ID24577617