Title | Particle display: a quantitative screening method for generating high-affinity aptamers. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Wang J, Gong Q, Maheshwari N, Eisenstein M, Arcila MLuz, Kosik KS, H Soh T |
Journal | Angew Chem Int Ed Engl |
Volume | 53 |
Issue | 19 |
Pagination | 4796-801 |
Date Published | 2014 May 5 |
ISSN | 1521-3773 |
Abstract | We report an aptamer discovery technology that reproducibly yields higher affinity aptamers in fewer rounds compared to conventional selection. Our method (termed particle display) transforms libraries of solution-phase aptamers into "aptamer particles", each displaying many copies of a single sequence on its surface. We then use fluorescence-activated cell sorting (FACS) to individually measure the relative affinities of >10(8) aptamer particles and sort them in a high-throughput manner. Through mathematical analysis, we identified experimental parameters that enable optimal screening, and demonstrate enrichment performance that exceeds the theoretical maximum achievable with conventional selection by many orders of magnitude. We used particle display to obtain high-affinity DNA aptamers for four different protein targets in three rounds, including proteins for which previous DNA aptamer selection efforts have been unsuccessful. We believe particle display offers an extraordinarily efficient mechanism for generating high-quality aptamers in a rapid and economic manner, towards accelerated exploration of the human proteome. |
DOI | 10.1002/anie.201309334 |
Alternate Journal | Angew. Chem. Int. Ed. Engl. |
PubMed ID | 24644057 |
PubMed Central ID | PMC4065591 |
Grant List | R01 AI085583 / AI / NIAID NIH HHS / United States R01A1076899 / / PHS HHS / United States U01 L099773-01 / / PHS HHS / United States U54 DK093467 / DK / NIDDK NIH HHS / United States U54 DK093467 / DK / NIDDK NIH HHS / United States |